Invention Grant
- Patent Title: Determination of variants produced upon replication or transcription of nucleic acid sequences
- Patent Title (中): 确定在核酸序列的复制或转录时产生的变体
-
Application No.: US11475921Application Date: 2006-06-28
-
Publication No.: US09150906B2Publication Date: 2015-10-06
- Inventor: Thuraiayah Vinayagamoorthy
- Applicant: Thuraiayah Vinayagamoorthy
- Applicant Address: CA Saskatchewan
- Assignee: BIO-ID DIAGNOSTIC INC.
- Current Assignee: BIO-ID DIAGNOSTIC INC.
- Current Assignee Address: CA Saskatchewan
- Agency: Morrison & Foerster LLP
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68

Abstract:
A method of determining whether or not a nucleic acid having an expected sequence or one or more variants of the expected sequence are present in a sample containing nucleic acids after replication, transcription or editing (or other transformation) of a substrate nucleic acid. The method involves deciding an expected sequence likely to be formed in the sample upon the replication, transcription or editing of the substrate nucleic acid, and possible variants of the expected sequence, providing primer pairs for a polymerase chain reaction, reverse transcriptase polymerase chain reaction or ligase chain reaction, carrying out the polymerase chain reaction or reverse transcriptase polymerase chain reaction in one or more steps to form amplicons, and analyzing the amplicons to determining whether or not a nucleic acid having the expected sequence and/or variants are present in the sample. The primers of the primer pairs are designed to anneal to regions of the nucleic acid of the expected sequence and the variants, the regions being selected to reveal unambiguously the presence or absence in the sample of the nucleic acid of the expected sequence or the variants thereof according to the presence or absence of specific amplicons amplified by the primers.
Public/Granted literature
- US20080003573A1 Determination of variants produced upon replication or transcription of nucleic acid sequences Public/Granted day:2008-01-03
Information query