Invention Grant
US09157105B2 Biosynthesis of CMP-legionaminic acid from fructose-6-P, and respective pathway intermediates, using novel GDP-linked precursors
有权
从果糖-6-P生物合成CMP-肉豆蔻酸，以及各自的途径中间体，使用新的GDP-前体
- Patent Title: Biosynthesis of CMP-legionaminic acid from fructose-6-P, and respective pathway intermediates, using novel GDP-linked precursors
- Patent Title (中): 从果糖-6-P生物合成CMP-肉豆蔻酸，以及各自的途径中间体，使用新的GDP-前体
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Application No.: US13140222Application Date: 2009-12-16
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Publication No.: US09157105B2Publication Date: 2015-10-13
- Inventor: Ian C. Schoenhofen , Susan M. Logan
- Applicant: Ian C. Schoenhofen , Susan M. Logan
- Applicant Address: CA Ottawa
- Assignee: National Research Council of Canada
- Current Assignee: National Research Council of Canada
- Current Assignee Address: CA Ottawa
- Agency: Cermak Nakajima & McGowan LLP
- Agent Malcolm K. McGowan
- International Application: PCT/CA2009/001800 WO 20091216
- International Announcement: WO2010/069047 WO 20100624
- Main IPC: C12P19/30
- IPC: C12P19/30 ; C12N9/90 ; C12N9/10 ; C12N9/12 ; C12N15/52 ; C12P19/28 ; C12P19/32
Abstract:
The sialic acid-like sugar legionaminic acid is found as a virulence-associated surface glyco-conjugate in Legionella pneumophila and Campylobacter coli. In this work, we have purified and biochemically characterized eleven candidate biosynthetic enzymes from C. jejuni, thereby fully reconstituting the biosynthesis of legionaminic acid and its CMP-activated form, starting from fructose-6-P. This pathway involves unique GDP-linked intermediates and provides a facile means for the efficient large-scale synthesis of an important sialic acid mimic and novel precursors.
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