Invention Grant
- Patent Title: Method of purifying factor VII and/or factor VIIa
- Patent Title (中): 纯化因子VII和/或因子VIIa的方法
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Application No.: US14205877Application Date: 2014-03-12
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Publication No.: US09175279B1Publication Date: 2015-11-03
- Inventor: Angela Anita Yvonne Michel , Ian Walker , Duy Dinh Nguyen
- Applicant: CSL Limited
- Applicant Address: AU Parkville, Victoria
- Assignee: CSL Limited
- Current Assignee: CSL Limited
- Current Assignee Address: AU Parkville, Victoria
- Agency: Finnegan, Henderson, Farabow, Garrett & Dunner, LLP
- Main IPC: C12N9/64
- IPC: C12N9/64 ; A61K38/48

Abstract:
The present invention relates generally to a method of purifying Factor VII and/or Factor VIIa from a solution containing either or both proteins, the method comprising: diluting the solution containing Factor VII and/or Factor VIIa in a loading buffer; adding the diluted solution from step (i) to a multi-modal anion exchange resinunder conditions selected such that Factor VII and/or Factor VIIa is bound to the resin; optionally washing the resin with a wash buffer; adding an elution buffer to the resin under conditions selected such that Factor VII and/or Factor VIIa is eluted from the resin; and recovering the eluted recombinant Factor VII and/or Factor VIIa; wherein the loading buffer, the optional wash buffer and the elution buffer each comprise about 35 mM or less of calcium ions.
Information query
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