Invention Grant
US09175284B2 Puro-DHFR quadrifunctional marker and its use in protein production
有权
Puro-DHFR四官能标记及其在蛋白质生产中的应用
- Patent Title: Puro-DHFR quadrifunctional marker and its use in protein production
- Patent Title (中): Puro-DHFR四官能标记及其在蛋白质生产中的应用
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Application No.: US14481983Application Date: 2014-09-10
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Publication No.: US09175284B2Publication Date: 2015-11-03
- Inventor: Michel Kobr , Philippe Dupraz
- Applicant: MERCK SERONO SA
- Applicant Address: CH Coinsins, Vaud
- Assignee: MERCK SERONO SA
- Current Assignee: MERCK SERONO SA
- Current Assignee Address: CH Coinsins, Vaud
- Agency: Salwanchik, Lloyd & Eisenschenk
- Priority: EP07109829 20070607
- Main IPC: C12N15/63
- IPC: C12N15/63 ; C12N15/85 ; C12N9/96 ; G01N33/74
Abstract:
This invention relates to industrial production of proteins. More specifically, the invention relates to the res-DHFR surrogate marker, which corresponds to a fusion between DHFR and a protein conferring resistance to a toxic compound or conferring a metabolic advantage. The invention further relates to the use of res-DHFR for screening cells for high expression of a protein of interest. The invention is illustrated by the Puro-DHFR surrogate marker, which corresponds to a fusion between the puromycin N-acetyltransferase and dihydrofolate reductase (DHFR).
Public/Granted literature
- US20150024485A1 PURO-DHFR QUADRIFUNCTIONAL MARKER AND ITS USE IN PROTEIN PRODUCTION Public/Granted day:2015-01-22
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