Invention Grant
US09175295B2 Modified transposon vector and its use 有权
改性转座子载体及其用途

Modified transposon vector and its use
Abstract:
A modified transposon vector and a method for introducing a foreign gene into a cell are provided. A modified transposon vector wherein a nucleic acid fragment having the following features (a)-(b) or (a)-(c) is inserted; a cell expressing a foreign gene obtained by introducing said vector into chromosomal DNA of a cell line or an ontogenetic cell and further introducing an expression cassette of a foreign gene into the chromosomal DNA; a genetically recombined animal generated by using said cell (in case of an ontogenetic cell); and a method for producing a foreign protein from said cell and said genetically recombined animal: (a) It consists of 5′- and 3′-TIR sequences of a transposon gene; (b) A sequence where a recombination reaction occurs is inserted between at least either of DR regions present by twos each in 5′- and 3′-TIR sequences of a transposon gene; (c) A restriction enzyme recognition site or an expression cassette of a foreign gene is inserted between 5′- and 3′-TIR sequences of a transposon gene.
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