Invention Grant
- Patent Title: Modified transposon vector and its use
- Patent Title (中): 改性转座子载体及其用途
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Application No.: US11994982Application Date: 2006-07-04
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Publication No.: US09175295B2Publication Date: 2015-11-03
- Inventor: Kazuyoshi Kaminaka , Hiroaki Maeda , Masaki Hirashima , Ryoichi Kawamura , Junichi Matsuda , Takashi Kuwana
- Applicant: Kazuyoshi Kaminaka , Hiroaki Maeda , Masaki Hirashima , Ryoichi Kawamura , Junichi Matsuda , Takashi Kuwana
- Applicant Address: JP Kumamoto
- Assignee: THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE
- Current Assignee: THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE
- Current Assignee Address: JP Kumamoto
- Agency: Browdy and Neimark, PLLC
- Priority: JP2005-196398 20050705; JP2005-196957 20050706
- International Application: PCT/JP2006/013302 WO 20060704
- International Announcement: WO2007/004642 WO 20070111
- Main IPC: C12N15/79
- IPC: C12N15/79 ; C12N5/10 ; C12N15/85

Abstract:
A modified transposon vector and a method for introducing a foreign gene into a cell are provided. A modified transposon vector wherein a nucleic acid fragment having the following features (a)-(b) or (a)-(c) is inserted; a cell expressing a foreign gene obtained by introducing said vector into chromosomal DNA of a cell line or an ontogenetic cell and further introducing an expression cassette of a foreign gene into the chromosomal DNA; a genetically recombined animal generated by using said cell (in case of an ontogenetic cell); and a method for producing a foreign protein from said cell and said genetically recombined animal: (a) It consists of 5′- and 3′-TIR sequences of a transposon gene; (b) A sequence where a recombination reaction occurs is inserted between at least either of DR regions present by twos each in 5′- and 3′-TIR sequences of a transposon gene; (c) A restriction enzyme recognition site or an expression cassette of a foreign gene is inserted between 5′- and 3′-TIR sequences of a transposon gene.
Public/Granted literature
- US20100281551A1 MODIFIED TRANSPOSON VECTOR AND ITS USE Public/Granted day:2010-11-04
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