Invention Grant
US09175326B2 Transglycosylation activity of glycosynthase mutants of an endo-beta-N-acetylglucosaminidase (endo-D) from streptococcus pneumoniae
有权
来自肺炎链球菌的内切-β-N-乙酰氨基葡萄糖苷酶（内切-D）的糖基转移酶突变体的转糖基化活性
- Patent Title: Transglycosylation activity of glycosynthase mutants of an endo-beta-N-acetylglucosaminidase (endo-D) from streptococcus pneumoniae
- Patent Title (中): 来自肺炎链球菌的内切-β-N-乙酰氨基葡萄糖苷酶（内切-D）的糖基转移酶突变体的转糖基化活性
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Application No.: US13759221Application Date: 2013-02-05
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Publication No.: US09175326B2Publication Date: 2015-11-03
- Inventor: Lai-Xi Wang
- Applicant: UNIVERSITY OF MARYLAND, BALTIMORE
- Applicant Address: US MD Baltimore
- Assignee: UNIVERSITY OF MARYLAND, BALTIMORE
- Current Assignee: UNIVERSITY OF MARYLAND, BALTIMORE
- Current Assignee Address: US MD Baltimore
- Agency: Moore & Van Allen, PLLC
- Agent Marianne Fuierer
- Main IPC: C12P21/06
- IPC: C12P21/06 ; C12P21/08 ; C12N9/24 ; A61K39/00 ; C07K1/00 ; C07K14/00 ; C07K16/00 ; C07K17/00 ; C12P21/00 ; C07K16/28
Abstract:
The present invention provides for recombinant Endo-D and selected mutants that exhibit reduced hydrolysis activity and increased transglycosylation activity for the synthesis of glycoproteins wherein a desired sugar chain is added to a core fucosylated or nonfucosylated GlcNAc-protein acceptor by transglycosylation. Such recombinant Endo-D and selected mutants are useful for efficient glycosylation remodeling of IgG1-Fc domain.
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