Invention Grant
US09175327B2 Composition for synthesizing protein with reduced lipopolysaccharide contamination, method for producing protein using said composition
有权
用于合成具有减少的脂多糖污染的蛋白质的组合物，使用所述组合物生产蛋白质的方法
- Patent Title: Composition for synthesizing protein with reduced lipopolysaccharide contamination, method for producing protein using said composition
- Patent Title (中): 用于合成具有减少的脂多糖污染的蛋白质的组合物，使用所述组合物生产蛋白质的方法
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Application No.: US13100050Application Date: 2011-05-03
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Publication No.: US09175327B2Publication Date: 2015-11-03
- Inventor: Takashi Kanamori , Yuki Hayami , Kanehisa Kojoh , Takuya Ueda , Kumiko Tsuihiji , Tomoe Fuse , Mikiko Nakamura , Shizue Kato
- Applicant: Takashi Kanamori , Yuki Hayami , Kanehisa Kojoh , Takuya Ueda , Kumiko Tsuihiji , Tomoe Fuse , Mikiko Nakamura , Shizue Kato
- Applicant Address: JP Kashiwa JP Tokyo
- Assignee: GeneFrontier Corporation,The University of Tokyo
- Current Assignee: GeneFrontier Corporation,The University of Tokyo
- Current Assignee Address: JP Kashiwa JP Tokyo
- Agency: Leydig, Voit & Mayer, Ltd.
- Priority: JP2011-053547 20110310
- Main IPC: C12P21/02
- IPC: C12P21/02 ; C12N15/10
Abstract:
According to the present invention, a composition possessing cell-free protein synthesis activity with reduced contaminating lipopolysaccharide, and a method for producing a protein using the same are provided. When ribosome display is performed using the composition and method for protein production of the present invention, the background that is caused by non-specific binding is reduced, so that a nucleic acid that encodes the desired polypeptide can be selected with high accuracy and high efficiency.
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