Invention Grant
- Patent Title: Methods for amplifying nucleic acid using tag-mediated displacement
- Patent Title (中): 使用标签介导位移扩增核酸的方法
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Application No.: US14344372Application Date: 2012-09-21
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Publication No.: US09175337B2Publication Date: 2015-11-03
- Inventor: Norman C. Nelson , Margarita Batranina-Kaminsky
- Applicant: Gen-Probe Incorporated
- Applicant Address: US CA San Diego
- Assignee: Gen-Probe Incorporated
- Current Assignee: Gen-Probe Incorporated
- Current Assignee Address: US CA San Diego
- Agent Jeffrey E. Landes; Nicholas V. Sherbina
- International Application: PCT/US2012/056666 WO 20120921
- International Announcement: WO2013/044097 WO 20130328
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H21/02

Abstract:
Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5′ to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5′ to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.
Public/Granted literature
- US20140370550A1 METHODS FOR AMPLIFYING NUCLEIC ACID USING TAG-MEDIATED DISPLACEMENT Public/Granted day:2014-12-18
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