Invention Grant
US09176114B2 Cell culture model for acquired chemoresistance of chronic myelogenous leukemia and related methods for identifying agents to overcome resistance
有权
用于慢性骨髓性白血病的获得性化学抗性的细胞培养模型和用于鉴定药物克服抗性的相关方法
- Patent Title: Cell culture model for acquired chemoresistance of chronic myelogenous leukemia and related methods for identifying agents to overcome resistance
- Patent Title (中): 用于慢性骨髓性白血病的获得性化学抗性的细胞培养模型和用于鉴定药物克服抗性的相关方法
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Application No.: US13922141Application Date: 2013-06-19
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Publication No.: US09176114B2Publication Date: 2015-11-03
- Inventor: WenYong Chen , Ravi Bhatia , Leila Su , Yate-Ching Yuan
- Applicant: CITY OF HOPE
- Applicant Address: US CA Duarte
- Assignee: CITY OF HOPE
- Current Assignee: CITY OF HOPE
- Current Assignee Address: US CA Duarte
- Agency: Perkins Coie LLP
- Agent Laura J. Dueppen
- Main IPC: A61K35/14
- IPC: A61K35/14 ; C12N5/0786 ; C12N5/09 ; C12P19/34 ; G01N33/50
Abstract:
A method of generating a chronic myelogenic leukemia (CML) acquired chemoresistant culture model is provided. Such a method may comprise providing a naïve blast crisis CML cell line; administering/contacting the cell line with a mutation-inducing dose of imatinib; maintaining a culture of the treated cell line for a period of time until the treated cell line relapses and repopulates the culture; and determining the repopulated cell culture is a CML acquired chemoresistant cell line by detecting a BCR-ABL mutation, wherein the acquired chemoresistance is achieved by a BCR-ABL mutation.
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