Invention Grant
US09176136B2 Hybridoma cell line ST03, monoclonal antibody against aflatoxin biosynthetic precursor sterigmatocystin and use thereof
有权
杂交瘤细胞系ST03，抗黄曲霉毒素生物合成前体灭菌素的单克隆抗体及其用途
- Patent Title: Hybridoma cell line ST03, monoclonal antibody against aflatoxin biosynthetic precursor sterigmatocystin and use thereof
- Patent Title (中): 杂交瘤细胞系ST03，抗黄曲霉毒素生物合成前体灭菌素的单克隆抗体及其用途
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Application No.: US14666869Application Date: 2015-03-24
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Publication No.: US09176136B2Publication Date: 2015-11-03
- Inventor: Peiwu Li , Min Li , Qi Zhang , Zhaowei Zhang , Xiaoxia Ding
- Applicant: OILCROPS RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURE SCIENCES
- Applicant Address: CN Wuhan, Hubei
- Assignee: OILCROPS RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURE SCIENCES
- Current Assignee: OILCROPS RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURE SCIENCES
- Current Assignee Address: CN Wuhan, Hubei
- Agency: Locke Lord LLP
- Agent Tim Tingkang Xia, Esq.
- Priority: CN201410115952 20140326
- Main IPC: C07K16/14
- IPC: C07K16/14 ; C07K16/00 ; C07K14/38 ; G01N33/569
Abstract:
A hybridoma cell line ST03 having China Center for Type Culture Collection (CCTCC) accession number C2013187, a monoclonal antibody against aflatoxin biosynthetic precursor ST produced by the hybridoma cell line ST03, and the use of the monoclonal antibody. The hybridoma cell line ST03 can be used for preparing a high-titer monoclonal antibody against aflatoxin biosynthetic precursor ST, and the titer of mouse ascites antibody against aflatoxin biosynthetic precursor ST determined by enzyme linked immunosorbent assay (ELISA) can reach 6.4×105. The monoclonal antibody against aflatoxin biosynthetic precursor ST has high sensitivity, has 50% inhibiting concentration IC50 to aflatoxin biosynthetic precursor ST of 0.36 ng/mL, has no cross reaction with all of aflatoxin B1, aflatoxin B2, aflatoxin G1, and aflatoxin G2, and can be used for the content determination of aflatoxin biosynthetic precursor ST.
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