Invention Grant
- Patent Title: Microscopic device and method for three-dimensional localization of point-like objects in a specimen
- Patent Title (中): 用于样品中点样物体三维定位的显微镜装置和方法
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Application No.: US13672881Application Date: 2012-11-09
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Publication No.: US09179131B2Publication Date: 2015-11-03
- Inventor: Jonas Foelling
- Applicant: Leica Microsystems CMS GmbH
- Applicant Address: DE Wetzlar
- Assignee: LEICA MICROSYSTEMS CMS GMBH
- Current Assignee: LEICA MICROSYSTEMS CMS GMBH
- Current Assignee Address: DE Wetzlar
- Agency: Leydig, Voit & Mayer, Ltd.
- Priority: DE102011055294 20111111
- Main IPC: H04N15/00
- IPC: H04N15/00 ; A61B1/06 ; H04N13/02 ; G02B27/00 ; G01N21/64 ; G02B21/16 ; G02B21/36 ; G02B27/58

Abstract:
A microscopic device for three-dimensional localization of point-like objects, encompassing a detection optical system that images point-like objects, each in the form of a three-dimensional focus light distribution, into an image space; a color separation apparatus that divides the light into at least two separate light bundles of different wavelength regions; at least two image space detector units , one receiving one light bundle and the other receiving the other light bundle, each detector unit comprising a light-spot-sensing detection surface ; an evaluation unit that ascertains a lateral X-Y position and an axial Z position relative to the sharpness plane in a direction perpendicular to the sharpness plane; at least one Z-position correction value for at least one of the wavelength regions being stored in the evaluation unit, which value indicates a detection optical system longitudinal chromatic aberration in that wavelength region; and the evaluation unit correcting the Z position.
Public/Granted literature
- US20130120539A1 MICROSCOPIC DEVICE AND METHOD FOR THREE-DIMENSIONAL LOCALIZATION OF POINT-LIKE OBJECTS IN A SPECIMEN Public/Granted day:2013-05-16
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