An oral appliance cleaner and holder is a device that is used to clean an oral appliance via scrubbing in conjunction with a liquid cleaning solution. The oral appliance is placed in between a first brush and a second brush. The first brush is able to rotate along with a rotatable base in order to scrub the oral appliance. The rotatable base is mounted to a fluid reservoir that transfers liquid cleaning solution to the first brush and the second brush. A lid and a sealing ring are used to prevent leakage of the liquid cleaning solution from the fluid reservoir. A plurality of slots is present in the first brush and the second brush to allow the liquid cleaning solution to pass from the fluid reservoir and come into contact with a plurality of bristles of the first brush and a plurality of bristles of the second brush.