Invention Grant
- Patent Title: Synthetic siRNA detection method
- Patent Title (中): 合成siRNA检测方法
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Application No.: US13186941Application Date: 2011-07-20
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Publication No.: US09200314B2Publication Date: 2015-12-01
- Inventor: Satoshi Yanagi , Eiji Kobayashi , Takashi Uemori , Hiroyuki Mukai
- Applicant: Satoshi Yanagi , Eiji Kobayashi , Takashi Uemori , Hiroyuki Mukai
- Applicant Address: JP Otsu-Shi, Shiga
- Assignee: TAKARA BIO INC.
- Current Assignee: TAKARA BIO INC.
- Current Assignee Address: JP Otsu-Shi, Shiga
- Agency: Banner & Witcoff, Ltd.
- Priority: JP2010-164109 20100721
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68 ; C12N15/10 ; C07H21/02 ; C07H21/04
Abstract:
A method for accurately and easily detecting a synthetic siRNA, for example, a siRNA in which the 3′ end is DNA, and a kit used for the method are provided. The present invention relates to a method for detecting a siRNA in which the 3′ end is DNA, comprising: (a) adding polydeoxyadenosine to the 3′ DNA end of at least one strand of the siRNA to be detected to produce a polydeoxyadenosine-added RNA; (b) annealing a polydeoxythymidine primer having a tag sequence at its 5′ side to the polydeoxyadenosine-added RNA and synthesizing DNA from the primer by a reverse transcription; and (c) detecting the DNA synthesized in (b).
Public/Granted literature
- US20120021420A1 Synthetic siRNA Detection Method Public/Granted day:2012-01-26
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