A hot plug structure of an electronic peripheral device has a connecting port. The hot plug structure includes a base having a linear way, a support connected to the base and moveable along the linear way in which the electronic peripheral device is fixed on the support, a connector installed on the base and disposed corresponding to one side of the linear way, and a rotating part disposed corresponding to the other side of the linear way. The rotating part has a first pivot portion pivoted around the base and a second pivot portion pivoted around the support. The rotating part can be rotated to drive the support close to or away from the connector such that the connecting port is plugged in or unplugged from the connector. Therefore, the connection efficiency and use convenience of the hot plug structure are improved.