Invention Grant
- Patent Title: Mutated rep encoding sequences for use in AAV production
- Patent Title (中): 用于AAV生产的突变的编码序列
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Application No.: US13583920Application Date: 2011-03-11
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Publication No.: US09228174B2Publication Date: 2016-01-05
- Inventor: Yvet Noordman , Jacek Lubelski , Andrew Christian Bakker
- Applicant: Yvet Noordman , Jacek Lubelski , Andrew Christian Bakker
- Applicant Address: NL Amsterdam
- Assignee: UNIQURE IP B.V.
- Current Assignee: UNIQURE IP B.V.
- Current Assignee Address: NL Amsterdam
- Agency: Foley & Lardner LLP
- Agent Sunit Talapatra
- International Application: PCT/NL2011/050170 WO 20110311
- International Announcement: WO2011/112089 WO 20110915
- Main IPC: C12N15/35
- IPC: C12N15/35 ; C12N5/10 ; C12N7/02 ; C12N15/85 ; C12N7/00 ; C12N15/86

Abstract:
Nucleic acids encoding Parvoviral Rep proteins with a mutated nuclear localization signal (NLS) are provided. Also provided is a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein with a mutated zinc finger domain and a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein comprising an amino acid mutation at position 43, 57, 79, 97, 120, 179, 305, 484, 493 or 571 with reference to SEQ ID NO: 2. Nucleic acid constructs and cells, such as insect cells, comprising the nucleic acids are provided as well as a method for producing a recombinant Parvoviral virion using the nucleic acids.
Public/Granted literature
- US20130023034A1 MUTATED REP ENCODING SEQUENCES FOR USE IN AAV PRODUCTION Public/Granted day:2013-01-24
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