Invention Grant
- Patent Title: Switchable gRNAs comprising aptamers
- Patent Title (中): 可切换的包含适体的gRNA
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Application No.: US14326329Application Date: 2014-07-08
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Publication No.: US09228207B2Publication Date: 2016-01-05
- Inventor: David R. Liu , Johnny Hao Hu
- Applicant: President and Fellows of Harvard College
- Applicant Address: US MA Cambridge
- Assignee: President and Fellows of Harvard College
- Current Assignee: President and Fellows of Harvard College
- Current Assignee Address: US MA Cambridge
- Agency: Wolf, Greenfield & Sacks, P.C.
- Main IPC: C12N15/113
- IPC: C12N15/113 ; C12N15/115 ; C12N15/90 ; C12N9/22 ; C12N15/01 ; A61K48/00
Abstract:
Some aspects of this disclosure provide compositions, methods, systems, and kits for controlling the activity and/or improving the specificity of RNA-programmable endonucleases, such as Cas9. For example, provided are guide RNAs (gRNAs) that are engineered to exist in an “on” or “off” state, which control the binding and hence cleavage activity of RNA-programmable endonucleases.
Public/Granted literature
- US20150071900A1 SWITCHABLE GRNAS COMPRISING APTAMERS Public/Granted day:2015-03-12
Information query
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