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US09228218B2 Expression vector for animal cells including CSP-B 5′-SAR factor and method for producing recombinant proteins using same 有权
包括CSP-B 5'-SAR因子在内的动物细胞的表达载体及使用其的重组蛋白质的制备方法

Expression vector for animal cells including CSP-B 5′-SAR factor and method for producing recombinant proteins using same
Abstract:
The present invention relates to an expression vector for animal cells, comprising: (a) CSP-B (Cytotoxic Serine Protease-B) 5′-SAR (Scaffold or Matrix Attachment Region); (b) a promoter operable in animal cells; and (c) a polyadenylation sequence, and to a method for producing recombinant proteins using same. The vector of the present invention includes CSP-B 5′-SAR, and thus has the effect of overcoming the inhibition of gene expression according to the position of a foreign gene introduced into an animal cell, and significantly improving the expression rate of a target protein. The vector of the present invention effectively expresses recombinant proteins for drugs or antibodies in animal cells. The vector of the present invention and the method for producing recombinant proteins using same may be very usefully applied to the industrial mass production of drugs.
Abstract(Chinese):

本发明涉及动物细胞的表达载体，其包含：（a）CSP-B（细胞毒性丝氨酸蛋白酶-B）5'-SAR（支架或基质附着区）; （b）可在动物细胞中操作的启动子; 和（c）聚腺苷酸化序列，以及使用其生产重组蛋白的方法。 本发明的载体包括CSP-B 5'-SAR，因此具有根据引入动物细胞的外源基因的位置克服基因表达的抑制的作用，并显着提高靶的表达率 蛋白。 本发明的载体在动物细胞中有效表达药物或抗体的重组蛋白质。 本发明的载体和使用其的重组蛋白质的制备方法可以非常有效地应用于药物的工业量产。

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