A scanning apparatus for scanning a biological sample labeled with fluorochrome includes a case, a sample table, a light source module, an image capture unit, and a driving unit. The light source module provides excitation light to the biological sample on the sample table, in which the fluorochrome is excited to generate fluorescence by fluorescence resonance energy transfer. The image capture unit catches images of the fluorescence from the biological sample. The driving unit is connected to the image capture unit to move the image capture unit. The present invention further includes a filter unit on the image capture unit to filter the fluorescence into the image capture unit.