Invention Grant
US09243043B2 Filamentous fungal mutants with improved homologous recombination efficiency
有权
具有改善的同源重组效率的丝状真菌突变体
- Patent Title: Filamentous fungal mutants with improved homologous recombination efficiency
- Patent Title (中): 具有改善的同源重组效率的丝状真菌突变体
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Application No.: US10594014Application Date: 2005-03-31
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Publication No.: US09243043B2Publication Date: 2016-01-26
- Inventor: Petrus Jacobus Theodorus Dekker , Marco Alexander van den Berg
- Applicant: Petrus Jacobus Theodorus Dekker , Marco Alexander van den Berg
- Applicant Address: NL
- Assignee: DSM IP ASSETS B.V.
- Current Assignee: DSM IP ASSETS B.V.
- Current Assignee Address: NL
- Agency: Miles & Stockbridge, P.C.
- Agent Susan E. Shaw McBee
- Priority: EP04076057 20040402
- International Application: PCT/EP2005/051464 WO 20050331
- International Announcement: WO2005/095624 WO 20051013
- Main IPC: C12N1/14
- IPC: C12N1/14 ; C12N15/00 ; C12Q1/68 ; C12N9/00 ; C12P21/04 ; C07H21/04 ; C07K1/00 ; C07K14/37 ; C12N15/80 ; C12N15/90 ; C12P21/02 ; C12P23/00 ; C12P37/00
Abstract:
The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions.
Public/Granted literature
- US20080227145A1 Filamentous Fungal Mutants With Improved Homologous Recombination Efficiency Public/Granted day:2008-09-18
Information query
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