Invention Grant
US09243242B2 Methods of making di-tagged DNA libraries from DNA or RNA using double-tagged oligonucleotides
有权
使用双标记寡核苷酸从DNA或RNA制备二标记DNA文库的方法
- Patent Title: Methods of making di-tagged DNA libraries from DNA or RNA using double-tagged oligonucleotides
- Patent Title (中): 使用双标记寡核苷酸从DNA或RNA制备二标记DNA文库的方法
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Application No.: US14226321Application Date: 2014-03-26
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Publication No.: US09243242B2Publication Date: 2016-01-26
- Inventor: Yan Wang
- Applicant: Yan Wang
- Agent Lili Chen
- Main IPC: C12N15/10
- IPC: C12N15/10 ; C12N15/65 ; C12N15/66 ; C12Q1/68
Abstract:
Disclosed are methods, compositions and kits related to making double-tagged DNA libraries from RNA/DNA samples. A double-tagged oligonucleotide (DTO) is employed to efficiently add two different tags to ends of DNAs to make a double-tagged DNA libraries. Also disclosed are methods to make mate pair libraries using the double-tagged oligonucleotide, and methods to make double-tagged single stranded DNA. The double-tagged DNA libraries of the invention are ready to be used on next generation sequencing machines.
Public/Granted literature
- US20140206581A1 Methods of Making Di-Tagged DNA Libraries from DNA or RNA Using Double-tagged Oligonucleotides Public/Granted day:2014-07-24
Information query
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