Invention Grant
- Patent Title: Enzyme preparation containing thermostable DNA polymerase, method for producing same, and method for detecting subject organism to be detected
- Patent Title (中): 含有热稳定性DNA聚合酶的酶制剂及其制造方法以及检测被检测体的方法
-
Application No.: US13144175Application Date: 2010-01-15
-
Publication No.: US09243272B2Publication Date: 2016-01-26
- Inventor: Homare Tabata , Hiroshi Minami , Hideki Niimi , Isao Kitajima , Tomohiro Ueno , Shiroh Hayashi , Masashi Mori
- Applicant: Homare Tabata , Hiroshi Minami , Hideki Niimi , Isao Kitajima , Tomohiro Ueno , Shiroh Hayashi , Masashi Mori
- Applicant Address: JP Hokkaido JP Toyama
- Assignee: HOKKAIDO MITSUI CHEMICALS INC.,NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMA
- Current Assignee: HOKKAIDO MITSUI CHEMICALS INC.,NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMA
- Current Assignee Address: JP Hokkaido JP Toyama
- Agency: Buchanan, Ingersoll & Rooney PC
- Priority: JP2009-006556 20090115; JP2009-023707 20090204; JP2009-040052 20090223; JP2009-181755 20090804
- International Application: PCT/JP2010/050443 WO 20100115
- International Announcement: WO2010/082640 WO 20100722
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34 ; C12N9/12
Abstract:
Disclosed is a thermostable DNA polymerase preparation which can illimitably reduce the risk of false positivity in the detection of a subject microorganism utilizing a gene amplification reaction and therefore enables the selective amplification of DNA for detecting the subject microorganism even when the amount of the subject microorganism is small and therefore the amount of DNA collected therefrom is extremely small, and can be produced at a reduced cost. Also disclosed is a method for quantifying or quantifying/identifying a subject organism to be detected rapidly, conveniently and with high sensitivity using the preparation of the present invention.
Public/Granted literature
Information query
