An add-in card fixing frame includes a frame body, one or more attached locking assemblies, and one or more limit switches. The frame body includes a first side plate, a second side plate, one or more separating plates, and one or more connecting portions. The separating plate includes one or more protruding part having an opening. The attached locking assembly includes a main plate, a top plate, and one or more side plates having a through hole and a locking member. When the add-in card is fixed, the locking member passes through a first screw hole, a second screw hole for fixing, and the add-in card is sandwiched between the top plate and the connecting portion. The top plate presses the limit switch for power connection. The attached locking assembly is fixed to the frame body, so use of hand tools is unnecessary. The screw falling out can be avoided.