A method, a device and a mobile terminal for 3D operation control of a touch screen are disclosed. The method comprises the following steps: acquiring capacitance values of at least two acquiring points in a motion trajectory formed by a finger or a conductor moving in a touch screen sensing space; calculating coordinates of the acquiring points on the X-axis, the Y-axis and the Z-axis respectively according to the capacitance values of the acquiring points to obtain 3D coordinates of the acquiring points; and determining the motion trajectory according to the 3D coordinates of the at least two acquiring points and executing a corresponding operation. In this way, the purpose of identifying a 3D gesture can be achieved to enrich the operations of the mobile terminal.