An optimal dynamic seam adjustment system for image stitching includes an image obtaining module, a feature difference calculation module and a dynamic seam module. The image obtaining module obtains at least two images to be stitched, which have at least one overlapping area divided into a plurality of pixels arranged in a plurality of pixel columns and a plurality of pixel rows. The feature difference calculation module calculates a feature difference value for each pixel within the overlapping area. The dynamic seam module establishes a plurality of dynamic seam routes started from a top end of the overlapping area, so as to find an optimal dynamic seam route based on the feature difference value, wherein each dynamic seam route is composed of a plurality of pixels.