An imaging system has a microscope having an objective lens and a projection device configured to project spatially modulated light in one of several preselected predetermined pattern through the objective lens and onto tissue. The system camera configured to record an image of the tissue through the microscope and objective lens as illuminated by the spatially modulated light, and an image processor having a memory with a routine for performing spatial Fourier analysis on the image of the tissue to recover spatial frequencies. The image processor also constructs a three dimensional model of the tissue, and performs fitting of at least absorbance and scattering parameters of voxels of the model to match the recovered spatial frequencies. The processor then displays tomographic slices of the three dimensional model.