The present invention discloses a patterned transparent conductive film based on the random grid, wherein the surface of the conductive film can be divided into a conductive region and an insulating region, with the conductive region having the grid made of metal. The grid of the conductive region, composed of the grid line of the conductive region, is a random grid having an irregular shape, and can avoid periodic shielding generated by the opaque metal grid lines and the periodic pixel unit of LCD. The patterned transparent conductive film provided by the present invention is composed of the irregular random grid, and therefore will not generate the Moire stripe. Besides, finally by naked eyes observation, the conductive region and the insulating region have exactly the same or close transmittance, and therefore will not generate the grayscale contrast.