An assembly machine for assembling disposable syringe-driven filter is provided, which includes a rack and an automatic controller arranged on the rack. The automatic controller is electrically connected with a rotating table, a first automatic loading mechanism, an automatic filter disc-sucking mechanism, a second automatic loading mechanism, and an automatic unloading mechanism. Using the first and second automatic loading mechanisms, the automatic filter disc-sucking mechanism, and the automatic unloading mechanism, as well as the automatic controller, the assembly machine can realize the automatic loading of the upper cover of a disposable syringe-driven filter, the automatic placing of filter disc, the automatic loading and assembling of the lower cover of filter and the automatic unloading of the disposable syringe-driven filter, so that the automatic production of the disposable syringe-driven filter can be realized.