Invention Grant
- Patent Title: Protease degradable polypeptides and uses thereof
- Patent Title (中): 蛋白酶降解多肽及其用途
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Application No.: US13990866Application Date: 2011-12-01
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Publication No.: US09265844B2Publication Date: 2016-02-23
- Inventor: Ching-Hsuan Tung , Wael R. Abd-Elgaliel , Craig D. Logsdon , Zobeida Cruz-Monserrate
- Applicant: Ching-Hsuan Tung , Wael R. Abd-Elgaliel , Craig D. Logsdon , Zobeida Cruz-Monserrate
- Applicant Address: US TX Houston US TX Austin
- Assignee: THE METHODIST HOSPITAL SYSTEM,BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
- Current Assignee: THE METHODIST HOSPITAL SYSTEM,BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
- Current Assignee Address: US TX Houston US TX Austin
- Agency: Kilpatrick Townsend & Stockton LLP
- International Application: PCT/US2011/062817 WO 20111201
- International Announcement: WO2012/075241 WO 20120607
- Main IPC: A61B5/00
- IPC: A61B5/00 ; A61B8/00 ; A61B10/00 ; C12Q1/37 ; A61K38/04 ; C07K5/00 ; C07K7/00 ; C07K16/00 ; C07K17/00 ; A61K49/00 ; A61K47/48 ; G01N33/533 ; G01N33/542 ; G01N33/574 ; G01N21/64 ; C12N15/11 ; A61K48/00 ; A61K38/00

Abstract:
Provided herein are polypeptides that are selectively cleaved by cathepsin E. Also provided are methods of detecting cathepsin E. The methods comprise contacting cathepsin E with the polypeptides provided herein and detecting fluorescence. Further provided are methods of diagnosing cancer or pre-cancerous conditions in a subject. Also provided herein is a multilayered nanoparticle or a composition comprising the multilayer nanoparticle, wherein the multilayered nanoparticle comprises a negatively charged nanoparticle core or capsule coated with alternating positive and negative layers. Optionally, the positive layer comprises a positively charged protease degradable polypeptide. Optionally, the negative layer comprises a negatively charged therapeutic agent or a therapeutic agent and a means for providing the agent with a negative charge. For example, optionally, the therapeutic agent is linked to a negatively charged polymer. Further provided are methods of treating or preventing a disease characterized by expression of a protease in a subject using the nanoparticle.
Public/Granted literature
- US20130251640A1 PROTEASE DEGRADABLE POLYPEPTIDES AND USES THEREOF Public/Granted day:2013-09-26
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