Invention Grant
US09273296B2 Meganuclease variants cleaving a DNA target sequence from a glutamine synthetase gene and uses thereof
有权
从谷氨酰胺合成酶基因切割DNA靶序列的大范围核酸酶变体及其用途
- Patent Title: Meganuclease variants cleaving a DNA target sequence from a glutamine synthetase gene and uses thereof
- Patent Title (中): 从谷氨酰胺合成酶基因切割DNA靶序列的大范围核酸酶变体及其用途
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Application No.: US13062795Application Date: 2008-09-08
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Publication No.: US09273296B2Publication Date: 2016-03-01
- Inventor: Julianne Smith
- Applicant: Julianne Smith
- Applicant Address: FR Romainville
- Assignee: CELLECTIS
- Current Assignee: CELLECTIS
- Current Assignee Address: FR Romainville
- Agency: Oblon, McClelland, Maier & Neustadt, L.L.P.
- International Application: PCT/IB2008/003109 WO 20080908
- International Announcement: WO2010/026443 WO 20100311
- Main IPC: C12N9/22
- IPC: C12N9/22
Abstract:
An I-CreI variant, wherein one of the two I-CreI monomers has at least two substitutions, one in each of the two functional subdomains of the LAGLIDADG core domain situated respectively from positions 28 to 40 and 44 to 77 of I-CreI, said variant being able to cleave a DNA target sequence from the glutamine synthetase gene. Use of said variant and derived products for improving expression system for the production of recombinant protein.
Public/Granted literature
- US20110225664A1 MEGANUCLEASE VARIANTS CLEAVING A DNA TARGET SEQUENCE FROM A GLUTAMINE SYNTHETASE GENE AND USES THEREOF Public/Granted day:2011-09-15
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