Invention Grant
- Patent Title: Serum-free stable transfection and production of recombinant human proteins in human cell lines
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Application No.: US14506107Application Date: 2014-10-03
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Publication No.: US09273325B2Publication Date: 2016-03-01
- Inventor: Carola Schroeder , Haiyan Ding , Cathleen Wegmann
- Applicant: Carola Schroeder , Haiyan Ding , Cathleen Wegmann
- Agency: Thompson Hine LLP
- Priority: EP05105965 20050630
- Main IPC: C12N15/85
- IPC: C12N15/85 ; C12N5/071 ; C12P21/02 ; C07K14/535 ; C07K14/755 ; C07K14/81 ; C12N9/64
Abstract:
The present invention relates to an improved method for the serum-free production of an immortalized human cell line stably transfected under serum-free conditions with a specific vector carrying the gene coding for the protein of interest. Furthermore the invention relates to a production cell line obtained by said method, a production method for said protein of interest utilizing said production cell line, and the specific vector carrying the gene of interest itself.
Public/Granted literature
- US20150044720A1 SERUM-FREE STABLE TRANSFECTION AND PRODUCTION OF RECOMBINANT HUMAN PROTEINS IN HUMAN CELL LINES Public/Granted day:2015-02-12
Information query
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