An electrosurgical system may generally first and second electrodes coupled to an energy source operative to generate and deliver pulses of a biphasic radio frequency (RF) waveform to treat undesirable tissue in a patient. The pulses may induce non-thermal cell death in the patient's tissue while causing no or minimal muscle contractions in the treated patient. The pulses may be grouped in bursts wherein the pulses within a burst repeat at a particular pulse frequency.