Invention Grant
- Patent Title: Methods for preparing an active TNFR-Fc fusion protein
- Patent Title (中): 制备活性TNFR-Fc融合蛋白的方法
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Application No.: US14239216Application Date: 2012-08-17
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Publication No.: US09279014B2Publication Date: 2016-03-08
- Inventor: Hye Soon Won , Byung Je Sung , Yong Ho Ahn , Sang Kyung Park
- Applicant: Hye Soon Won , Byung Je Sung , Yong Ho Ahn , Sang Kyung Park
- Applicant Address: CH Aubonne
- Assignee: ARES TRADING S.A.
- Current Assignee: ARES TRADING S.A.
- Current Assignee Address: CH Aubonne
- Agency: Lathrop & Gage LLP
- Agent Brian C. Trinique
- Priority: KR10-2011-0081854 20110817
- International Application: PCT/KR2012/006564 WO 20120817
- International Announcement: WO2013/025079 WO 20130221
- Main IPC: C07K14/705
- IPC: C07K14/705 ; C07K14/715
Abstract:
The present invention relates to a method for separating and preparing a TNFR-Fc fusion protein using hydrophobic interaction chromatography (HIC). More particularly, the present invention relates to a method for separating and preparing a highly pure active protein from clipped proteins due to displacement effect by adjusting the conductivity of a protein sample using a high concentration of salt solution and by adjusting a loading amount thereof, and a TNFR-Fc fusion protein prepared by the method.
Public/Granted literature
- US20140316114A1 METHOD FOR PREPARING ACTIVE FORM OF TNFR-FC FUSION PROTEIN Public/Granted day:2014-10-23
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