A release sheet comprising a base material and a release agent layer formed on at least one surface of the base material by using a release agent composition; the release agent composition contains a first polydimethylsiloxane having at least two alkenyl groups in one molecule and a second polydimethylsiloxane having at least two hydrosilyl groups in one molecule; the release agent composition contains 0.40 to 5.25 mmol of the alkenyl groups and 7.00 to 120.00 mmol of the hydrosilyl groups in a total of 30 g of the first polydimethylsiloxane and the second polydimethylsiloxane; and a molar ratio (b/a) of the hydrosilyl groups (b) to the alkenyl groups (a) is 3.0 to 70.0. Consequently, it is possible to suppress the peeling force from increasing even when the release agent layer of the release sheet is exposed to the air for a long time.