Invention Grant
US09279112B2 Cellulase enzymes having a modified linker and reduced lignin binding
有权
具有改性接头和减少木质素结合的纤维素酶
- Patent Title: Cellulase enzymes having a modified linker and reduced lignin binding
- Patent Title (中): 具有改性接头和减少木质素结合的纤维素酶
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Application No.: US12713516Application Date: 2010-02-26
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Publication No.: US09279112B2Publication Date: 2016-03-08
- Inventor: Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
- Applicant: Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
- Applicant Address: CA Ontario
- Assignee: IOGEN ENERGY CORPORATION
- Current Assignee: IOGEN ENERGY CORPORATION
- Current Assignee Address: CA Ontario
- Agency: Fitzpatrick, Cella, Harper & Scinto
- Main IPC: C12N9/42
- IPC: C12N9/42 ; C12P19/14

Abstract:
Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acid sequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolyzing cellulose with the modified cellulases in the presence of lignin.
Public/Granted literature
- US20100221778A1 NOVEL LIGNIN-RESISTANT CELLULASE ENZYMES Public/Granted day:2010-09-02
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