Disclosed are an anti-influenza nucleic acid, peptide nucleic acid and preparations thereof. The nucleic acid of the invention consists of any one or several of SEQ ID NOs: 1-12, and any one or several of SEQ ID NOs: 13-24. The peptide nucleic acid of the invention consists of any one or several of SEQ ID NOs: 25-36, and any one or several of SEQ ID NOs: 37-48. The peptide nucleic acid preparation of the invention has effective ingredients of said peptide nucleic acids. The anti-influenza peptide nucleic acid of the invention is of high bioavailability and stable physical and chemical properties with no toxic or side effect and no drug tolerance. With low cost, the drugs can inhibit influenza virus replication and have good anti-virus effect.