There are provided a pixel unit driving circuit and driving method, pixel unit and display apparatus. The pixel unit driving circuit is used for driving a light-emitting device to emit light, and comprises a first thin film transistor (T1), a second thin film transistor (T2), a third thin film transistor (T3) and a storage capacitor (Cs). A gate of the first thin film transistor (T1) is connected with a control line (Gate), a first electrode thereof is connected with a data line (Data), and a second electrode thereof is connected with a first node (A). One gate of the second thin film transistor (T2) is connected with the control line (Gate) and the other gate is connected with a second scan line (Scan2), a first electrode thereof is connected with the storage capacitor (Cs), and a second electrode thereof is connected with a second node (B). One gate of the third thin film transistor (T3) is connected with the first node (A) and the other gate is connected with the second scan line (Scan2), a first electrode thereof is connected with a power supply (Vdd), and a second electrode thereof is connected with the second node (B). One terminal of the storage capacitor is connected with the first node (A), and the other terminal is connected with the first electrode of the second thin film transistor (T2). One terminal of the light-emitting device is connected with the second node (B), and the other terminal thereof is grounded. The pixel unit driving circuit can reduce an influence on a driving voltage caused by variations in threshold voltage.