Apparatus and techniques can include using one or more of a portable or fixed optical system to obtain images of a desired anatomical target, such as the fundus region of a human eye. The optical system can include one or more of a laser-based focusing target, a laser-based fixation target, or a removable or adjustable alignment assembly. Such an alignment assembly can provide or allow user alignment based on using anatomical landmarks other than the fundus being imaged. One or more of the apparatus or techniques can be implemented in an optical assembly included as a portion hand-held fundus camera, such as including or using a consumer or commercial digital camera to capture an image.