Provided is a detection circuit for dark points on a panel, comprising a preset voltage generation module and a selection circuit module, wherein the preset voltage generation module is connected with the selection circuit module and is used for transferring N preset voltages to the selection circuit module, and the selection circuit module comprises N inputting terminals for receiving the N preset voltages, respectively, an outputting terminal and a strobe switch for strobing the outputting terminal with one of the N inputting terminals, N≧2. The circuit according to the embodiments of the present disclosure can detect the dark point defect caused by remains in active layers effectively in a panel state and prevent the panels having such dark point defects from being incorporated into modules, so that a waste of cost is avoid and a detection capability in a detection procedure for the panel is increased.