The present subject matter provides a dishwasher appliance that measures the amount of ozone in a wash chamber of the appliance. The appliance is also configured for adjusting the amount of ozone in the wash chamber based upon such measurements. The appliance may adjust the amount of ozone by, e.g., adjusting the flow rate of wash fluid to the wash chamber, adjusting the flow rate of air in the wash chamber, and/or adjusting the temperature of wash chamber. Related methods are also provided.