Invention Grant
- Patent Title: Preparation of an esterase
- Patent Title (中): 酯酶的制备
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Application No.: US12667414Application Date: 2008-07-04
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Publication No.: US09309503B2Publication Date: 2016-04-12
- Inventor: Martin Kietzmann , Helmut Schwab , Harald Pichler , Mirela Ivancic , Oliver May , Rudolf Gijsbertus Marie Luiten
- Applicant: Martin Kietzmann , Helmut Schwab , Harald Pichler , Mirela Ivancic , Oliver May , Rudolf Gijsbertus Marie Luiten
- Applicant Address: NL Amsterdam
- Assignee: DPx Holdings B.V.
- Current Assignee: DPx Holdings B.V.
- Current Assignee Address: NL Amsterdam
- Agency: Pierce Atwood LLP
- Agent Raymond G. Arner
- Priority: EP07013092 20070704; EP08000828 20080117
- International Application: PCT/EP2008/058731 WO 20080704
- International Announcement: WO2009/004093 WO 20090108
- Main IPC: C12P21/02
- IPC: C12P21/02 ; C12N9/18 ; C12N15/70

Abstract:
The present invention relates to a recombinant E. coli strain characterized in that the organisms contains a gene coding for a protein having esterase activity. Such E. coli is suitable for the preparation of a protein with esterase activity, whereby the expression takes place without coexpression of GroEL and/or GroES from a plasmid. The expression of glutathione reductase and/or thioredoxin reductase may be abolished by mutation. Furthermore, the ability of the organism to grow under oxygen-rich conditions has been restored by mutation. The expression of the gene of the protein with esterase activity is unaccompanied by the expression of an additional gene encoding a heat shock chaperone protein.
Public/Granted literature
- US20110177553A1 PREPARATION OF AN ESTERASE Public/Granted day:2011-07-21
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