Invention Grant
US09309575B2 Mutant strains of Escherichia coli, a method of testing potential antibacterial agents using said strains as well as a testing kit
有权
大肠杆菌的突变菌株，使用所述菌株测试潜在抗菌剂的方法以及检测试剂盒
- Patent Title: Mutant strains of Escherichia coli, a method of testing potential antibacterial agents using said strains as well as a testing kit
- Patent Title (中): 大肠杆菌的突变菌株，使用所述菌株测试潜在抗菌剂的方法以及检测试剂盒
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Application No.: US13642121Application Date: 2011-04-23
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Publication No.: US09309575B2Publication Date: 2016-04-12
- Inventor: Piotr Koprowski , Andrzej Kubalski , Wojciech Grajkowski
- Applicant: Piotr Koprowski , Andrzej Kubalski , Wojciech Grajkowski
- Applicant Address: PL Warszawa
- Assignee: Instytut Biologii Doswiadczalnej IM. M. Nenckiego Pan
- Current Assignee: Instytut Biologii Doswiadczalnej IM. M. Nenckiego Pan
- Current Assignee Address: PL Warszawa
- Agency: Booth Udall Fuller, PLC
- Agent Rodney J. Fuller
- Priority: PL391045 20100423
- International Application: PCT/PL2011/050010 WO 20110423
- International Announcement: WO2011/133056 WO 20111027
- Main IPC: C12N1/21
- IPC: C12N1/21 ; C12Q1/18 ; C12R1/19 ; C07K14/245 ; C12N1/20
Abstract:
The subject of the present invention are mutant strains of Escherichia coli MG6165 (ATCC nr 47076) lacking all potassium transporters: Kdp, Kup and Trk (ΔKtrans), encompassing strains lacking at least one of the genes encoding mechanically gated channels mscS and mscL (ΔKtrans ΔmscS i/lub ΔmscL). In particular, it concerns Escherichia coli MG61655 (ATCC No. 47076) lacking all potassium transporters: Kdp, Kup and Trk (ΔKtrans), which additionally are devoid of the mechanically gated channel gene mscS—E. coli (ΔKtrans ΔmscS), the mechanically gated channel gene mscL—E. coli (ΔKtrans ΔmscL) and/or both the mechanically gated channel genes mscS and mscL—E. coli (ΔKtrans ΔmscS ΔmscL). The subject of the present invention is also a method of testing chemical compounds for potential antibacterial properties, making use of the abovementioned strains. Another subject of the present invention is a kit for testing chemical compounds as potential antibacterial substances, containing the abovementioned strains.
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