A biomedical testing sheet comprises: a substrate with calligraphy paper material, wherein the substrate comprises a wax pattern layer, which is a part of a surface of the substrate coated by a waxy material, and penetrated and diffused by the waxy material, and the wax pattern layer comprises one or more carrying units for carrying one or more droplets of liquids to be measured. The lotus effect substantially prevents diffusion of the liquids and reagents on the carrying units, and reduces the required amount of liquids to be measured and the amount of agent.