A motherboard with an electrostatic discharge protection (ESD) function including a first electrode, a second electrode, an isolation region and an energy storage unit is disclosed. The first electrode receives a grounding level. The second electrode includes at least one solder pad to fix an input/output port thereon. The isolation region is disposed between the first and the second electrodes. The energy storage unit is coupled between the first and the second electrodes and disposed across the isolation region.