Invention Grant
US09322053B2 Compositions and methods for monitoring in real time a nicking amplification reaction comprising a primer oligonucleotide with a 3′—terminal region comprising A2′—modified nucleotide
有权
用于实时监测包含具有包含A2'-修饰的核苷酸的3'末端区域的引物寡核苷酸的切口扩增反应的组合物和方法
- Patent Title: Compositions and methods for monitoring in real time a nicking amplification reaction comprising a primer oligonucleotide with a 3′—terminal region comprising A2′—modified nucleotide
- Patent Title (中): 用于实时监测包含具有包含A2'-修饰的核苷酸的3'末端区域的引物寡核苷酸的切口扩增反应的组合物和方法
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Application No.: US14789545Application Date: 2015-07-01
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Publication No.: US09322053B2Publication Date: 2016-04-26
- Inventor: Daniel Shaffer , Stephen A. Judice
- Applicant: ENVIROLOGIX, INC.
- Applicant Address: US ME Portland
- Assignee: EnviroLogix Inc.
- Current Assignee: EnviroLogix Inc.
- Current Assignee Address: US ME Portland
- Agency: Saul Ewing, LLP
- Agent Melissa Hunter-Ensor; Elbert C. Chiang
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68
Abstract:
The present invention features compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time comprising one or more primer oligonucleotides comprising a 5′ nicking enzyme recognition site and a 3′-terminal region comprising a 2′-modified nucleotide. These methods are compatible with amplification of target oligonucleotides in a test sample, including biological samples, using a nicking amplification reaction.
Public/Granted literature
- US20150315635A1 COMPOSITIONS AND METHODS FOR QUANTIFYING A NUCLEIC ACID SEQUENCE IN A SAMPLE Public/Granted day:2015-11-05
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