Methods of forming a pattern in a semiconductor device may be provided. The methods may include sequentially forming a first hard mask layer and a second hard mask layer on an etching target layer including first and second regions, forming a first spacer layer on the second hard mask layer, forming a second hard mask pattern layer by etching the second hard mask layer using the first spacer layer, forming a second spacer layer on a sidewall of the second hard mask pattern layer, forming a first hard mask pattern layer by etching the first hard mask layer using the second spacer layer, and etching the etching target layer using the first hard mask pattern layer.