The invention belongs to the technical field of displaying, and provides a composition, an alignment layer and a method for preparing the same, a liquid crystal (LC) alignment unit and a liquid crystal display panel. The invention can solve the problems existing in the prior methods for achieving of multi-domain display of LC display devices, such as complex manufacturing processes, high cost, difficult to be manufactured. The composition (alignment layer) of the present invention allows adjusting the alignment of the molecules in the surface of the resultant coating layer of the composition, through adjusting the contents of the components in the composition, the polymerization temperature, and/or the thickness of the coating of the composition. The alignment layer, the LC alignment unit, and the LC display panel according to the invention can be prepared using a simple process and allow to achieve a multi-domain display at a lower cost.