A method of assigning a frequency channel using efficient spectrum sensing in a multiple Frequency Assignment system includes a channel sensing process, wherein a base station of a secondary user classifies multiple frequency channels licensed to a primary user into a plurality of channel state sets according to usage states, periodically senses channel states by changing a sensing period according to the types of classified channel state sets, and manages the sensed channel states as frequency channel state set information; a channel state set broadcasting process; and a channel assigning process. Accordingly, a frequency channel can be assigned to a secondary user requesting channel assignment using frequency channel state set information without a primary user experiencing interference noise.