Invention Grant
- Patent Title: Histidine engineered light chain antibodies and genetically modified non-human animals for generating the same
- Patent Title (中): 组氨酸工程轻链抗体和基因修饰的非人类动物产生相同的
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Application No.: US14705916Application Date: 2015-05-06
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Publication No.: US09332742B2Publication Date: 2016-05-10
- Inventor: John McWhirter , Lynn MacDonald , Andrew J. Murphy
- Applicant: Regeneron Pharmaceuticals, Inc.
- Applicant Address: US NY Tarrytown
- Assignee: Regeneron Pharmaceuticals, Inc.
- Current Assignee: Regeneron Pharmaceuticals, Inc.
- Current Assignee Address: US NY Tarrytown
- Agency: Brownstein Hyatt Farber Schreck, LLP
- Agent Rita S. Wu; Margarita Zippin
- Main IPC: A01K67/027
- IPC: A01K67/027 ; C07K16/00 ; C12N15/85
Abstract:
A genetically modified non-human animal is provided, wherein the non-human animal expresses an antibody repertoire capable of pH dependent binding to antigens upon immunization. A genetically modified non-human animal is provided that expresses human immunoglobulin light chain variable domains derived from a limited repertoire of human immunoglobulin light chain variable gene segments that comprise histidine modifications in their germline sequence. Methods of making non-human animals that express antibodies comprising histidine residues encoded by histidine codons introduced into immunoglobulin light chain nucleotide sequences are provided.
Public/Granted literature
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