A bicycle training stand includes a frame, a first clamping unit, and a second clamping unit. An axle of a bicycle wheel is clamped between the first and the second clamping units. The first clamping unit includes an abutting member and an operation member, wherein the abutting member is fitted in an axle bore of the frame, and can be moved in an axial direction of the axle bore. The abutting member has an abutting end contacting the axle of the bicycle wheel. The operation member is pivotally connected to the frame, and can be moved between a first position and a second position. When the operation member is at the first position, the contacting portion presses the abutting member; when the operation member is at the second position, the contacting portion has no contact with the abutting member.