Invention Grant
- Patent Title: Nucleic acid extraction device, and nucleic acid extraction method, nucleic acid extraction kit, and nucleic acid extraction apparatus, each using the same
- Patent Title (中): 核酸提取装置,核酸提取方法,核酸提取试剂盒和核酸提取装置,各自使用
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Application No.: US14062228Application Date: 2013-10-24
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Publication No.: US09347056B2Publication Date: 2016-05-24
- Inventor: Yuji Saito , Fumio Takagi
- Applicant: Seiko Epson Corporation
- Applicant Address: JP
- Assignee: Seiko Epson Corporation
- Current Assignee: Seiko Epson Corporation
- Current Assignee Address: JP
- Agency: Harness, Dickey & Pierce, P.L.C.
- Priority: JP2012-237068 20121026; JP2012-237069 20121026
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12N15/10 ; B01D15/00 ; G01N30/60 ; B01L3/00 ; B01D15/18 ; B01D15/22 ; G01N30/00

Abstract:
A nucleic acid extraction device includes a tube that is internally provided with, in the following order, a first plug composed of a first oil, a second plug composed of a first washing liquid, which is phase-separated from an oil and is used for washing a nucleic acid-binding solid-phase carrier having nucleic acids bound thereto, a third plug composed of a second oil, a fourth plug composed of a reverse transcription reaction solution, which is phase-separated from an oil and is used for performing a reverse transcription reaction, a fifth plug composed of a third oil, a sixth plug composed of an eluent, which is phase-separated from an oil and is used for eluting the nucleic acids from the nucleic acid-binding solid-phase carrier having nucleic acids bound thereto, and a seventh plug composed of a fourth oil.
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